Molecular Cloning and Characterization of Dectin-1 Receptor cDNA Expressed on Macrophages of Buffalo (Bubalus bubalis)
نویسندگان
چکیده
Dectin-1 a member of C-type lectin family of pathogen recognition receptor is primarily expressed on dendritic cells and macrophages. In this study, dectin-1 receptor cDNA has been characterized using Bubalus bubalis macrophage RNA and primers designed from known dectin-1 sequences of cattle and sheep. cDNA was synthesized using reverse transcriptase and used for amplification of two Bubalus bubalis dectin-1 overlapping fragments of length 410 & 619 bp. The overlapping sequence from 189-410 was removed and the Open reading frame (ORF) was analyzed. Analysis of dectin-1 ORF sequence reveals that it has 97.9 percent of homology at nucleotide level and 96.5, 96.0 percent homology at amino acids level with Bos taurus dectin-1 AY937382, BC102340 congeners respectively. ORF of both species consist of 606 nucleotides encoding a protein of 201 amino acids. Buffalo dectin-1 shows 13 nucleotide substitutions whereas 7 and 8 amino acid substitutions when compared with Bos taurus AY937382, BC102340 congeners respectively. Phylogenetic tree analysis of the available dectin-1 sequences shows that the dectin-1 of buffalo and cattle are closely related.
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